Biosafety and biosecurity are disciplines that developed tools to prevent health hazards for humans, animals and plants. In TM5, the theoretical principles of biosecurity and biosafety are introduced, followed by a discussion on their application within a One Health approach, supported by concrete case studies. Participants will visit the Spiez Laboratory, which specialises in highly infectious diseases and engage in real-life, hands-on exercises. The module is taught in collaboration with the Swiss Biosecurity Network (SBNet).